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Sonication DH98-IIIDN is a multi-functional and multi-purpose instrument that uses strong ultrasound to produce cavitation effects in liquids and ultrasonically treat substances. It can be used to break a variety of animal and plant cells and virus cells. It can also be used for emulsification, separation, and homogenization. Chemical, extraction, defoaming, cleaning and accelerating chemical reactions, etc. It is widely used in biochemistry, microbiology, medicinal chemistry, surface chemistry, physics, zoology and other fields.

◆Used for crushing animal and plant cells, bacteria, spores or tissues, extracting protein from cells and conducting scientific culture experiments of viruses and vaccines.
◆Accelerate the reaction speed of chemistry and physics and accelerate the degassing of liquid.
◆Scientific analysis of crude oil dilution, oil-water emulsification, accelerated decrystallization, and glass homogenization.
◆Disperse rare earths, various inorganic minerals, and prepare a homogenized mixture of nearly one percent of nanometer latex, etc.
◆Quick, powerful and high-precision lotion for mold micro-holes and blind holes.

Experiment purpose
1. Understand the basic principles of ultrasonic cell disruption
2. Master the basic technology of ultrasonic cell disruption

Experimental principle
When the strong sound wave acts on the solution, the bubble generation, growth and breaking are related to the cavitation phenomenon. The shock wave and the scissors force caused by the cavitation phenomenon cause the cells to lyse.

Materials and reagents
Bacteria 10ml, beaker, shaved ice, 75% alcohol cotton ball.

Φ15mm           200ml－500ml
Φ20mm           500ml－1000ml
TIP DIAMETER            VOLUME
Φ2mm                        150ul－5ml
Φ3mm                        200ul－10ml
Φ6mm                        10ml－50ml
Φ10mm                      50ml－350ml
Φ15mm                      200ml－500ml
Φ20mm                      500ml－1000ml